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OpenSource
leverjs
Commits
abbbe2f7
Commit
abbbe2f7
authored
Sep 17, 2023
by
ac_fx
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SSF.drawKymosCondition
parent
84181332
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matlab/+SSF/drawKymosCondition.m
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matlab/+SSF/drawKymosCondition.m
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abbbe2f7
% % ROOT = '/g/leverjs/Olivier/Agne/march_2023_20x/';
% ROOT = '/g/leverjs/Olivier/Agne/2022-06-03_48h-depr-transcr-drugs/';
% % ROOT = '/g/leverjs/Olivier/Agne/2023-03-25_DHB-proliferation-setup2/';
% kymo_dir = fullfile(ROOT,'kymo_downsize');
% flist = dir(fullfile(kymo_dir,'*.LEVER'));
%
% % for treatment (tx)
% tblClasses = readtable('../transcr-drugs/expDescr-2022-06-03.xlsx');
% classList = tblClasses.Cell_Type;
% % for steady state (ss)
% % classList = get_ssClasses(flist);
% % for DHB
% % tblClasses = readtable('../DHB/expDescr-2023-03-25.csv');
% % classList = tblClasses.Cell_Type;
%
% % outfile = 'dhb_erk_kymos_condition.pdf';
% outfile = 'tx_erk_kymos_condition.pdf';
%
% cbLabel = 'ERK SSF';
% targetChannels = 1;
function
drawKymosCondition
(
flist
,
classList
,
labelNames
,
outfile
,
cbLabel
,
targetChannels
,
stride
)
xClasses
=
unique
(
classList
);
nKeep
=
0
;
clipRange
=
[
2.5
,
97.5
];
clipLimits
=
SSF
.
getClipLimits
(
flist
,
targetChannels
,
clipRange
,
nKeep
);
xyim
=
[];
% one per flist
for
cc
=
1
:
length
(
xClasses
)
idxCC
=
find
(
strcmp
(
classList
,
xClasses
(
cc
)));
flistCC
=
flist
(
idxCC
);
clf
;
imCC
=
{};
for
ff
=
1
:
length
(
flistCC
)
fname
=
fullfile
(
flistCC
(
ff
)
.
folder
,
flistCC
(
ff
)
.
name
);
imq
=
SSF
.
draw_ssf_kymo
(
fname
,
targetChannels
,
clipLimits
,
nKeep
);
szim
(
ff
,:)
=
size
(
imq
);
imq
(:,
1
)
=
0
;
imq
(:,
end
)
=
0
;
imq
(
1
:
2
,:)
=
0
;
imq
(
end
-
1
:
end
,:)
=
0
;
row
=
ceil
(
ff
/
stride
);
col
=
1
+
mod
(
ff
-
1
,
stride
);
if
1
==
col
imCC
{
row
}
=
[];
elseif
size
(
imq
,
1
)
~=
size
(
imCC
{
row
},
1
)
imq
=
imresize
(
imq
,[
size
(
imCC
{
row
},
1
),
NaN
]);
end
imCC
{
row
}
=
[
imCC
{
row
},
imq
];
end
sz
=
cellfun
(
@
size
,
imCC
,
'UniformOutput'
,
false
);
sz
=
vertcat
(
sz
{:});
if
size
(
sz
,
1
)
>
1
sz
=
max
(
sz
);
end
for
i
=
1
:
length
(
imCC
)
if
size
(
imCC
{
i
},
2
)
<
sz
(
2
)
imCC
{
i
}(
end
,
sz
(
2
))
=
0
;
end
end
ccLabels
=
labelNames
(
idxCC
);
imMontage
=
vertcat
(
imCC
{:});
clf
;
imagesc
(
imMontage
)
;
hold
on
axis
off
% label movies
for
ff
=
1
:
length
(
flistCC
)
row
=
ceil
(
ff
/
stride
);
col
=
1
+
mod
(
ff
-
1
,
stride
);
if
1
==
col
x
=
1
;
end
y
=
(
row
-
1
)
*
szim
(
ff
,
1
);
plot
([
x
,
x
+
szim
(
ff
,
2
)],[
y
,
y
],
'-k'
);
plot
([
x
,
x
+
szim
(
ff
,
2
)],[
y
+
szim
(
ff
,
1
),
y
+
szim
(
ff
,
1
)],
'-k'
);
plot
([
x
,
x
],[
y
,
y
+
szim
(
ff
,
1
)],
'-k'
)
% don't draw to your right...
% plot([x+szim(ff,2),x+szim(ff,2)],[y,y+szim(ff,1)],'-k')
text
(
x
,
y
,
ccLabels
{
ff
},
'Interpreter'
,
'none'
,
'FontSize'
,
6
,
'BackgroundColor'
,[
0.8
,
0.8
,
0.8
,
0.5
],
...
'VerticalAlignment'
,
'top'
);
x
=
x
+
szim
(
ff
,
2
);
% date_movie = regexp(flistCC(ff).name,'(\d{4}-\d{2}-\d{2}).+_(\d{2})','tokens');
% text(x,y,[date_movie{1}{1} '_' date_movie{1}{2}],'Interpreter','none','FontSize',6,'BackgroundColor',[0.8,0.8,0.8,0.5]);
% plot([x+62,x+62],[y+5,y+size(imq,1)-5],'-r')
% treatment = regexp(tblCC.Treatment{ff},'(.*)\w*(','tokens');
% text(x+30,y+20,[date_movie{1}{1} '_' date_movie{1}{2} ' :: ' treatment{1}{1}],'Interpreter','none','FontSize',6,'BackgroundColor',[0.8,0.8,0.8,0.7]);
end
% set background (index 0, colormap(1)) to white
cm
=
parula
();
cm
(
1
,:)
=
[
1
,
1
,
1
];
colormap
(
cm
);
axis
on
set
(
gca
,
'xtick'
,[]);
set
(
gca
,
'ytick'
,[]);
ylabel
(
'xy \Downarrow x'
);
xlabel
(
'time'
);
fx
=
strfind
(
flist
(
ff
)
.
name
,
'.LEVER'
);
fx
=
fx
(
1
)
-
1
;
title
(
xClasses
{
cc
},
'Interpreter'
,
'none'
);
tick_labels
=
round
(
linspace
(
clipLimits
(
1
),
clipLimits
(
2
),
5
),
2
);
cb
=
colorbar
(
'Ticks'
,[
1
,
64
,
128
,
192
,
255
],
'TickLabels'
,
tick_labels
);
cb
.
Label
.
String
=
cbLabel
;
if
1
==
cc
exportgraphics
(
gcf
,
outfile
);
else
exportgraphics
(
gcf
,
outfile
,
'Append'
,
true
);
end
end
\ No newline at end of file
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