... | @@ -8,14 +8,10 @@ However, for full validation it is often more convenient to directly specify the |
... | @@ -8,14 +8,10 @@ However, for full validation it is often more convenient to directly specify the |
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1. Select the root progenitor cell for an entire cell lineage (this is usually in the first frame).
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1. Select the root progenitor cell for an entire cell lineage (this is usually in the first frame).
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2. Choose the Edit -> Identify tree mitoses menu option.
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2. Choose the Edit -> Identify tree mitoses menu option.
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After selecting the mitosis identification interface all cell tracking overlays will disappear except the selected cell.
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After selecting the mitosis identification interface all cell tracking overlays will disappear except the selected cell.
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3. Scroll or watch the movie until the selected cell undergoes mitosis.
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3. Scroll or watch the movie until the selected cell undergoes mitosis.
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4. In the first frame where the cell has split, click and drag to draw a line between the centers of the two daughter cells.
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4. In the first frame where the cell has split, click and drag to draw a line between the centers of the two daughter cells.
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When the mouse button is released a new mitotic event will be added to the tree and the two daughter cells should be identified by colored outlines. One of the two daughters will be automatically selected and outlined in red.
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When the mouse button is released a new mitotic event will be added to the tree and the two daughter cells should be identified by colored outlines. One of the two daughters will be automatically selected and outlined in red.
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5. Follow the selected cell until it undergoes mitosis
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5. Follow the selected cell until it undergoes mitosis
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6. Click and drag the red line between the center of the two daughter cells to identify the mitotic event.
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6. Click and drag the red line between the center of the two daughter cells to identify the mitotic event.
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7. Repeat steps 5-6 until all mitotic events in the clone have been identified.
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7. Repeat steps 5-6 until all mitotic events in the clone have been identified.
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