Copyright 2014 Andrew Cohen, Eric Wait, and Mark Winter
This file is part of LEVER 3-D - the tool for 5-D stem cell segmentation, tracking, and lineaging. See [Bioimage](http://bioimage.coe.drexel.edu/info/?page_id=620] for details. LEVER 3-D is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version. LEVER 3-D is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with LEVer in file "gnu gpl v3.txt". If not, see GNU.
NVIDIA CUDA toolkit EULA can be found at CUDA EULA
To run LEVER 3-D executable you will need the following: MATLAB's runtime called MCR version 8.3 (R2014a) Visual C++ Redistributable for Visual Studio 2013
DirectX 11 runtime (for Windows 7, no need on Windows 8) and capable device DirectX 11
To process images you will need: A CUDA compute 3.5 capable device or better
Once all of the above prerequisites have been met, run the LEVER3D.exe from the same directory as:
DefaultMeshShaders.fx
ViewAlignedVertexShader.fx
The program will open and ask for a metadata file. Please see example dataset here
The metadata needs to be in the same directory as the tiff files.
The tiff files need to be named such that the following sprintf line will find
each file sprintf('%s_c%02d_t%04d_z%04d.tif',datasetName,channel,frame,zSlice)
Please see 'Additional Files 3' of BMC paper for a video tutorial of the user interface.