| 
 | 
 | 
# General Editing
 | 
| 
 | 
 | 
# LEVER General Editing Interface
 | 
| 
 | 
 | 
---
 | 
| 
 | 
 | 
#### Right Click Menu Controls - Cells Window
 | 
| 
 | 
 | 
Additional options that appear when the user right-clicks on a hull in the Cells User Interface
 | 
| 
 | 
 | 
 | 
| 
 | 
 | 
In depth examples of the LEVER general editing interface are published in the LEVER protocol:
 | 
| 
 | 
 | 
 | 
| 
 | 
 | 
Winter et al., _Vertebrate neural stem cell segmentation, tracking and lineaging with validation and editing_, Nat Protocols, vol. 6, pp. 1942-1952, 2011.
 | 
| 
 | 
 | 
 | 
| 
 | 
 | 
#### Mouse Controls
 | 
| 
 | 
 | 
|Control |  Description |
 | 
| 
 | 
 | 
| ----   |------ |
 | 
| 
 | 
 | 
|Add Mitosis | Takes the current Hull/Track and adds it to the Family of another as a sibling |
 | 
| 
 | 
 | 
|Change Label | Allows the user to change the track association of a Hull|
 | 
| 
 | 
 | 
|Change Parents | |
 | 
| 
 | 
 | 
|Change number of Parents| |
 | 
| 
 | 
 | 
|Remove Cell(This Frame)|  |
 | 
| 
 | 
 | 
|Remove from Tree | |
 | 
| 
 | 
 | 
|Properties | |
 | 
| 
 | 
 | 
|Phenotype| Allows the user to select a "Phenotype" to assign to the Hull||
 | 
| 
 | 
 | 
|Scroll wheel| Scrolls through the movie |
 | 
| 
 | 
 | 
|Middle click| Splits a selected cell into two components or searches for a new detection if no cell selected |
 | 
| 
 | 
 | 
|Click a cell| Select the cell and update the tree window with the selected cell lineage |
 | 
| 
 | 
 | 
|Ctrl + click| Select multiple cells for merge or deletion |
 | 
| 
 | 
 | 
|Click and drag <br> onto another cell| Swaps the label of two cells to fix a tracking error||
 | 
| 
 | 
 | 
---
 | 
| 
 | 
 | 
#### Right Click Menu Controls - Lineage Window
 | 
| 
 | 
 | 
Additional options that appear when the user right-clicks on a hull in the Tree User Interface
 | 
| 
 | 
 | 
 | 
| 
 | 
 | 
#### Right Click Menu Controls - Cells Window
 | 
| 
 | 
 | 
|Control |  Description |
 | 
| 
 | 
 | 
| ----   |------ |
 | 
| 
 | 
 | 
|Remove Mitosis | Takes the current Hull/Track and adds it to the Family of another as a sibling |
 | 
| 
 | 
 | 
|Add Mitosis | Takes the current Hull/Track and adds it to the Family of another as a sibling|
 | 
| 
 | 
 | 
|Change Parents | |
 | 
| 
 | 
 | 
|Properties|Opens a window to show the user values such as HullID, FamilyID, Fist and Last Frame, etc|
 | 
| 
 | 
 | 
|Add Mitosis| Create a new mitotic event with the selected cell as one of the daughters |
 | 
| 
 | 
 | 
|Change Label| Change the label of the selected cell beginning at the current time |
 | 
| 
 | 
 | 
|Change number of Cells| Splits the selected cell detection into the specified number of regions |
 | 
| 
 | 
 | 
|Remove Cell (This Frame)| Delete a single erroneous cell detection |
 | 
| 
 | 
 | 
|Properties| Display properties about the selected cell |
 | 
| 
 | 
 | 
|Phenotype| Allows the user to assign a "Phenotype" to the Hull||
 | 
| 
 | 
 | 
---
 | 
| 
 | 
 | 
#### Mouse Controls
 | 
| 
 | 
 | 
#### Right Click Menu Controls - Lineage Window
 | 
| 
 | 
 | 
|Control |  Description |
 | 
| 
 | 
 | 
| ----   |------ |
 | 
| 
 | 
 | 
|Dragging a Hull | Allows user to switch the label(Track association) of two hulls|
 | 
| 
 | 
 | 
|Scrolling Wheel | Changes the Current Time  |
 | 
| 
 | 
 | 
|Clicking on Wheel | Splits a hull into two Hulls using Gaussian Mixture Model ||
 | 
| 
 | 
 | 
|Remove Mitosis| Remove the selected (erroneously identified) mitotic event |
 | 
| 
 | 
 | 
|Add Mitosis| Create a new mitotic event with the selected cell as the parent |
 | 
| 
 | 
 | 
|Properties| Display properties about the selected cell |
 | 
| 
 | 
 | 
---
 | 
| 
 | 
 | 
#### Keyboard Commands
 | 
| 
 | 
 | 
|Control |  Description |
 | 
| 
 | 
 | 
| ----   |------ |
 | 
| 
 | 
 | 
|Ctrl + O|Opens a prompt to allow the user to select a different dataset or mat file |
 | 
| 
 | 
 | 
|Ctrl + W|Closes the figures, if the data is unsaved, asks user to save|
 | 
| 
 | 
 | 
|Ctrl + S|Saves updates to the mat file |
 | 
| 
 | 
 | 
|Ctrl + Z|Undoes previous action |
 | 
| 
 | 
 | 
|Ctrl + Y|Undoes the previous Undo |
 | 
| 
 | 
 | 
|Ctrl + U|Prevents automatic mitosis edits to be made to tracks in the current family |
 | 
| 
 | 
 | 
|Ctrl + R|Prevents automatic segmentation edits to be made to hulls in the current family|
 | 
| 
 | 
 | 
|Ctrl + L|Toggles the labels in the Cell window of Hulls in the current tree |
 | 
| 
 | 
 | 
|Ctrl + B| |
 | 
| 
 | 
 | 
|Ctrl + I|Toggles the render of the Image in the Cell Window |
 | 
| 
 | 
 | 
|Ctrl + N|Switches through the different fluorescent channels, if exists |
 | 
| 
 | 
 | 
|Ctrl + P|Moves the images forward in time |
 | 
| 
 | 
 | 
|Ctrl + T|Makes the Tree with most tracks the current and displays it on Lineage window |
 | 
| 
 | 
 | 
|Ctrl + F|Allows the user to enter a Family id to make current |
 | 
| 
 | 
 | 
|Enter   |Merges together all of the currently selected Hulls ||
 | 
| 
 | 
 | 
---
 | 
| 
 | 
 | 
 | 
| 
 | 
 | 
**The original LEVER software protocol with usage instructions for analyzing proliferating cells was published in Nature Protocols. Read for more information**
 | 
| 
 | 
 | 
 | 
| 
 | 
 | 
Winter et al., Vertebrate neural stem cell segmentation, tracking and lineaging with validation and editing, Nat Protocols, vol. 6, pp. 1942-1952, 2011. | 
| 
 | 
 | 
|Ctrl + O| Open a new LEVER data file |
 | 
| 
 | 
 | 
|Ctrl + W| Close LEVER |
 | 
| 
 | 
 | 
|Ctrl + S| Saves changes LEVER data file |
 | 
| 
 | 
 | 
|Ctrl + Z| Undo the last edit |
 | 
| 
 | 
 | 
|Ctrl + Y| Redo a previously undone edit |
 | 
| 
 | 
 | 
|Ctrl + U| Lock tree: Prevent automated processing from changing tree structure |
 | 
| 
 | 
 | 
|Ctrl + R| Freeze tree: Preventing any automated changes to any cell on the tree |
 | 
| 
 | 
 | 
|Ctrl + L| Show labels: Toggle the display of cell labels |
 | 
| 
 | 
 | 
|Ctrl + P| Play the movie |
 | 
| 
 | 
 | 
|Ctrl + T| Go to frame |
 | 
| 
 | 
 | 
|Ctrl + F| Find cell by label |
 | 
| 
 | 
 | 
|Space   | Play the movie |
 | 
| 
 | 
 | 
|Delete  | Remove the selected cells |
 | 
| 
 | 
 | 
|Enter   | Merges all selected cells into a single component || |